Part:BBa_K3692004:Design

SAG1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 55
  Illegal PstI site found at 540
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 55
  Illegal PstI site found at 540
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 73
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 55
  Illegal PstI site found at 540
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 55
  Illegal PstI site found at 540
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 493
  

Design Notes

_

Source

AGCGCCAAAAGCTCTTTTATCTCAACCACTACTACTGATTTAACAAGTATAAACACTAGTGCGTATTCCACTGGATCCATTTCCACAGTAGAAACAGGCAATCGAACTACATCAGAAGTGATCAGCCATGTGGTGACTACCAGCACAAAACTGTCTCCAACTGCTACTACCAGCCTGACAATTGCACAAACCAGTATCTATTCTACTGACTCAAATATCACAGTAGGAACAGATATTCACACCACATCAGAAGTGATTAGTGATGTGGAAACCATTAGCAGAGAAACAGCTTCGACCGTTGTAGCCGCTCCAACCTCAACAACTGGATGGACAGGCGCTATGAATACTTACATCTCGCAATTTACATCCTCTTCTTTCGCAACAATCAACAGCACACCAATAATCTCTTCATCAGCAGTATTTGAAACCTCAGATGCTTCAATTGTCAATGTGCACACTGAAAATATCACGAATACTGCTGCTGTTCCATCTGAAGAGCCCACTTTTGTAAATGCCACGAGAAACTCCTTAAATTCCTTCTGCAGCAGCAAACAGCCATCCAGTCCCTCATCTTATACGTCTTCCCCACTCGTATCGTCCCTCTCCGTAAGCAAAACATTACTAAGCACCAGTTTTACGCCTTCTGTGCCAACATCTAATACATATATCAAAACGAAAAATACGGGTTACTTTGAGCACACGGCTTTGACAACATCTTCAGTTGGCCTTAATTCTTTTAGTGAAACAGCAGTCTCATCTCAGGGAACGAAAATTGACACCTTTTTAGTGTCATCCTTGATCGCATATCCTTCTTCTGCATCAGGAAGCCAATTGTCCGGTATCCAACAGAATTTCACATCAACTTCTCTCATGATTTCAACCTATGAAGGTAAAGCGTCTATATTTTTCTCAGCTGAGCTCGGTTCGATCATTTTTCTGCTTTTGTCGTACCTGCTATTCTAA