Coding

Part:BBa_K3692004:Design

Designed by: Juli Mukhadze   Group: iGEM20_Estonia_TUIT   (2020-10-07)


SAG1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 73
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 493


Design Notes

_


Source

AGCGCCAAAAGCTCTTTTATCTCAACCACTACTACTGATTTAACAAGTATAAACACTAGTGCGTATTCCACTGGATCCATTTCCACAGTAGAAACAGGCAATCGAACTACATCAGAAGTGATCAGCCATGTGGTGACTACCAGCACAAAACTGTCTCCAACTGCTACTACCAGCCTGACAATTGCACAAACCAGTATCTATTCTACTGACTCAAATATCACAGTAGGAACAGATATTCACACCACATCAGAAGTGATTAGTGATGTGGAAACCATTAGCAGAGAAACAGCTTCGACCGTTGTAGCCGCTCCAACCTCAACAACTGGATGGACAGGCGCTATGAATACTTACATCTCGCAATTTACATCCTCTTCTTTCGCAACAATCAACAGCACACCAATAATCTCTTCATCAGCAGTATTTGAAACCTCAGATGCTTCAATTGTCAATGTGCACACTGAAAATATCACGAATACTGCTGCTGTTCCATCTGAAGAGCCCACTTTTGTAAATGCCACGAGAAACTCCTTAAATTCCTTCTGCAGCAGCAAACAGCCATCCAGTCCCTCATCTTATACGTCTTCCCCACTCGTATCGTCCCTCTCCGTAAGCAAAACATTACTAAGCACCAGTTTTACGCCTTCTGTGCCAACATCTAATACATATATCAAAACGAAAAATACGGGTTACTTTGAGCACACGGCTTTGACAACATCTTCAGTTGGCCTTAATTCTTTTAGTGAAACAGCAGTCTCATCTCAGGGAACGAAAATTGACACCTTTTTAGTGTCATCCTTGATCGCATATCCTTCTTCTGCATCAGGAAGCCAATTGTCCGGTATCCAACAGAATTTCACATCAACTTCTCTCATGATTTCAACCTATGAAGGTAAAGCGTCTATATTTTTCTCAGCTGAGCTCGGTTCGATCATTTTTCTGCTTTTGTCGTACCTGCTATTCTAA

References